Anatolijs aripo
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Anatoly Sharipo, PhD
Latvian Biomedical Research and Study Centre
Ratsupites iela 1
Riga, LV 1067
LatviaPhone: + 371 7808007
Fax: + 371 7428038
Mobile: +371-9142807
E-mail: sharip@latnet.lv ; asla@latnet.lv
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Born: February 5, 1962, Silene, Daugavpils, Latvia
Languages: Latvian, Russian, English
Scientific Interests
- Adenovirus Oncogenes, Adenovirus Expression vectors (Aivars Vilnis, MI, Latvia,
1983-1986)
- Baculovirus Sructural Proteins, Baculovirus Expression vectors (V.Solomko, MI, Kiev,
Ukraine; G.Eglite, BI, Latvia, 1986-1992)
- Bacillus thuringiensis endotoxins (Dr. Rituma, Latvian Agricultural Academy,
1988-1994)
- Epstein-Barr virus antigens: presentation of virus epitopes for T-cell immune responce
(Maria Masucci, MTC, KI, Sweden, 1994-2000)
- Ubiquitin-Proteasomal degradation of viral and cellular
proteins (Maria Masucci, MTC, Magnus Ingelman-Sundberg, IMM, KI, Sweden, Pauls
Pumpens, BMC, LU, Latvia, 1994-2003)
- Protein fnolding (Souren Mkrtchian, IMM, Sweden, Gottfried Otting, UA, Australia,
Nicholas Dixon, UA, Australia, 1996-2003)
- Artificial Protein Design (Gottfried Otting, UA, Australia, Mats Persson, KI, Sweden,
1999-2003)
Education
- 1984 BSc, Faculty of Biology, University of Latvia, Riga, Latvia
- 1983-84 Biochemistry and molecular biology of proteins - training course at Russian
Academy of Sciences, Pushchino, Russia
- 1991 PhD thesis, Institute of Viral Biopreparates, Moscow
- 1994 Nostrification (Dr.biol.) at Latvian Academy of Sciences
- 1996 Protein Structure, Course by Professor C.-I. Branden at Karolinska Institute,
Sweden
Professional Experience
- 1984-1993: Research assistant/Researcher, Kirchenstein Institute of Microbiology,
Latvian Academy of Sciences
- 1993-2000: Head of Laboratory of Applied Microbiology , Kirchenstein Institute of
Microbiology and Virology, Riga, Latvia
- 1994-2000: Guest researcher at MTC, Karolinska Institute, Sweden, (see home page at MTC,
KI)
- 2000-2002: Guest researcher at IMM, Karolinska Institute, Sweden (see home page at IMM,
KI)
- Since 2000: Principal Investigator, Group leader, Biomedicine Reaserch and Study Centre,
University of Latvia, Riga, Latvia
Honours and Awards
Professional Activities and Memberships
- Since 2002: Vice-Chairman of Scientific Council, Biomedicine Research and Study Centre,
University of Latvia
- Since 2003: Member of Latvian Council of Science Expert Commission for Biotechnology,
Microbiology, Molecular Biology and Virology
Students
- 1989 Ainars Leonchiks, Diploma Project at LU, Latvia
- 1994 Ginta Pece, Diploma Project at RTI, Latvia
- 1998 Olga Matvijenko, MSci Program at LU, Latvia
- 2000-2002 Ainars Leonchiks, PhD program, co-supervising at Karolinska Institute, Sweden
- 2001-present Marina Koulikovska, PhD program, co-supervising at Linkopping
University, Sweden
- 2002, Raitis Barbals, BSci Program at LU, Latvia
- 2002, Karina Derkacha, BSci Program at LU, Latvia
- 2003, Raitis Barbals, MSci Program at LU, Latvia
- 2003, Karina Derkacha, MSci Program at LU, Latvia
Representative Publications (PubMed referred, high impact factor only)
- G.Pintacuda, A.Moshref, A.Leonchiks, A.Sharipo, G.Otting. Site-specific
labelling with a metal chelator for protein-structure refinement. - J.Biomol. NMR, 2004,
vol. 29, N 3, pp. 351-361.
- Liepinsh E, Barbals R, Dahl E, Sharipo A, Staub E, Otting G. The
Death-domain Fold of the ASC PYRIN Domain, Presenting a Basis for PYRIN/PYRIN Recognition.
- J. Mol. Biol. , 2003 Oct 3; 332 (5): 1155-63.
- Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G. Solution
structure of the R3H domain from human Smubp-2. - J. Mol. Biol. , 2003 Feb 7; 326
(1): 217-23.
- Dantuma NP, Sharipo A, Masucci MG. Avoiding proteasomal processing: the
case of EBNA1. - Curr. Top. Microbiol. Immunol., 2002; 269: 23-36. Review.
- Leonchiks A, Stavropoulou V, Sharipo A, Masucci MG. Inhibition of
ubiquitin-dependent proteolysis by a synthetic glycine-alanine repeat peptide that mimics
an inhibitory viral sequence. FEBS Lett. 2002 Jul 3;522(1-3):93-8.
- Sargsyan E, Baryshev M, Backlund M, Sharipo A, Mkrtchian S. Genomic
organization and promoter characterization of the gene encoding a putative endoplasmic
reticulum chaperone, ERp29. Gene. 2002 Feb 20;285(1-2):127-39.
- Li L, Sharipo A, Chaves-Olarte E, Masucci MG, Levitsky V, Thelestam M,
Frisan T. The Haemophilus ducreyi cytolethal distending toxin activates sensors of DNA
damage and repair complexes in proliferating and non-proliferating cells.Cell Microbiol.
2002 Feb;4(2):87-99.
- Sargsyan E, Baryshev M, Szekely L, Sharipo A, Mkrtchian S.
Identification of ERp29, an endoplasmic reticulum lumenal protein, as a new member of the
thyroglobulin folding complex. J Biol Chem. 2002 May 10;277(19):17009-15. Epub 2002 Mar
07.
- Heessen S, Leonchiks A, Issaeva N, Sharipo A, Selivanova G, Masucci MG,
Dantuma NP. Functional p53 chimeras containing the Epstein-Barr virus Gly-Ala repeat are
protected from Mdm2- and HPV-E6-induced proteolysis. Proc Natl Acad Sci U S A. 2002 Feb
5;99(3):1532-7. Epub 2002 Jan 22.
- Williams NK, Prosselkov P, Liepinsh E, Line I, Sharipo A, Littler DR,
Curmi PM, Otting G, Dixon NE. In vivo protein cyclization promoted by a circularly
permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem. 2002 Mar
8;277(10):7790-8. Epub 2001 Dec 12.
- Liepinsh E, Baryshev M, Sharipo A, Ingelman-Sundberg M, Otting G,
MkrtchianS. Thioredoxin fold as homodimerization module in the putative chaperone
ERp29: NMR structures of the domains and experimental model of the 51 kDa dimer. Structure
(Camb). 2001 Jun;9(6):457-71.
- Sharipo A, Imreh M, Leonchiks A, Branden C, Masucci MG. cis-Inhibition
of proteasomal degradation by viral repeats: impact of length and amino acid composition.
FEBS Lett. 2001 Jun 15;499(1-2):137-42.
- Leonchiks A, Liepinsh E, Barishev M, Sharipo A, Masucci MG, Otting
G. Random coil conformation of a Gly/Ala-rich insert in IkappaB alpha excludes
structural stabilization as the mechanism for protection against proteasomal degradation.
FEBS Lett. 1998 Dec 4;440(3):365-9.
- Mkrtchian S, Baryshev M, Matvijenko O, Sharipo A, Sandalova T,
Schneider G, Ingelman-Sundberg M, Mkrtchian S. Oligomerization properties of ERp29, an
endoplasmic reticulum stress protein. FEBS Lett. 1998 Jul 24;431(3):322-6. Erratum in:
FEBS Lett 1998 Aug 21;433(3):335.
- Sharipo A, Imreh M, Leonchiks A, Imreh S, Masucci MG. A minimal
glycine-alanine repeat prevents the interaction of ubiquitinated IkappaB alpha with the
proteasome: a new mechanism for selective inhibition of proteolysis. Nature Med. 1998
Aug;4(8):939-44.
- Levitskaya J, Sharipo A, Leonchiks A, Ciechanover A, Masucci MG.
Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat
domain of the Epstein-Barr virus nuclear antigen 1. Proc Natl Acad Sci U S A,.
1997 Nov 11; 94 (23): 12616-21.
Research
projects and collaborations
- Gly-Ala repeat of EBV nuclear antigen: influence of ubiquitin-proteasomal degradation:
Maria G. Masucci, MTC, Karolinska Institute, Sweden
- ERp29 escort protein of endoplasmic reticulum: Souren Mkrtchian, IMM, Karolinska
Institute, Sweden
- Cytochrome P450 2E1: Mechanisms of stability and protein turnover: Magnus
Ingelman-Sundberg, IMM, Karolinska Institute, Sweden
- C-ret Drug discovery, 5th framework of EU projects: Carlos Ibanez, Neurology,
Karolinska Institute, Sweden
- Proteasomal degradation and wounds in cornea: Per Fagerholm, Linkopping University,
Linkopping, Sweden
- Refolding of protein domain structures: Gottfried Otting, University of Australia,
Canberra, Australia
- Intein mediated in vitro protein ligation: Nick Dixon, University of Australia,
Canberra, Australia
- Mechanisms of HBcAg stability in vivo: Pauls Pumpens, BMC, LU, Riga, Latvia
Last update 12.04.2007